rabbit anti arpc5  (Novus Biologicals)

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The expression levels of APRC5 in different cancers, normal tissues, and cells. (A) The differential expression of ARPC5 in pan-cancer tissues from TCGA datasets. (B) The differential expression of ARPC5 in pan-cancer tissues based on TCGA and GTEx datasets. (C) ARPC5 expression in paired cancer tissues and adjacent normal tissues from TCGA datasets. (D) The mRNA expression levels of ARPC5 in different normal tissues from HPA database. (E) The mRNA expression of ARPC5 in cancer cell lines from HPA database. (F) ARPC5 mRNA expression in different single cell types from HPA database. ns: no significance; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing, Quantitative Proteomics

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Genetic alteration of ARPC5 in pan-cancer. (A) Mutation type and mutation frequency of ARPC5 obtained from the cBioPortal website. (B) The expression levels of ARPC5 in various CNV status of pan-cancer, CNV, copy number variations; *p < 0 . 0 5 ; * *p < 0.01; ****p < 0.0001.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Mutagenesis, Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Prognosis analyses of ARPC5 in pan-cancer based on univariate Cox regression method. (A) The correlation between ARPC5 expression and OS. (B) The correlation between ARPC5 expression and PFI. (C) The correlation between ARPC5 expression and DSS. OS: overall survival; PFI: progression-free interval; DSS: disease-specific survival.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: ARPC5 expression significantly correlated with OS based on Kaplan–Meier analysis. (A) The correlation in ESCA. (B) The correlation in HNSC. (C) The correlation in KIRC. (D) The correlation in KIRP. (E) The correlation in LIHC. (F) The correlation in LGG. (G) The correlation in OV. (H) The correlation in SKCM. The optimal cutoff of ARPC5 expression were used to divide patients into high- and low-expression groups.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 expression and clinical stage, histologic grade, and tumor molecular subtypes in various cancers based on Spearman’s correlation analysis (the correlation with p < 0.05 were displayed). (A) The correlation between ARPC5 expression and clinical stage in pan-cancer. (B–C) The expression levels of ARPC5 in different clinical stages of KIRC (B) and KIRP (C) . (D) The correlation between ARPC2 expression and histologic grade in pan-cancer. (E–H) The expression levels of ARPC5 in different histologic grades of KIRC (E) , LGG (F) , LIHC (G) , and UCEC (H) . (I–R) The correlation between ARPC5 expression and molecular subtypes in ACC (I) , BRCA (J) , LGG (K) , HNSC (L) , KIRP (M) , OV (N) , LUSC (O) , PCPG (P) , STAD (Q) , UCEC (R) . rho; rank coefficient of Spearman. Pv; p -value. NS, no significance.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 expression and immune scores and stromal scores of tumor microenvironments in pan-cancer.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 and immune infiltration cells in pan-cancer based on TIMER algorithm. (A) Heatmap displayed the correlation between ARPC5 expression and the proportions of B cell, CD4 + T cell, CD8 + T cell, neutrophil, macrophage, and DC cell. (B) The top five cancer types (including KIRC, LGG, PRAD, THCA, and THYM) with most significant correlation between ARPC5 and immune infiltration cells were displayed with scatterplots. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The correlation between ARPC5 expression and immune subtypes in pan-cancer using TISIDB. The cancers with significant correlation were displayed. (A) In BLCA. (B) In BRCA. (C) In CESC. (D) In KICH. (E) In KIRC. (F) In LGG. (G) In LIHC. (H) In LUAD. (I) In PAAD. (J) In OV. (K) PCPG. (L) PRAD. (M) In READ. ( N) In SARC. (O) In SKCM. (P) In STAD. (Q) In TGCT. (R) In THCA. (S) In UCS. (T) In UCEC. Pv; p -value. C1, wound healing; C2, IFN-gamma dominant; C3, inflammatory; C4, lymphocyte depleted; C5, immunologically quiet; C6, TGF-b dominant.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: The relationship between ARPC5 and immune-checkmate inhibitors biomarkers in pan-cancer. (A) The heatmap showing the co-expression relationship between ARPC5 and 47 immune checkpoint–related genes. (B) Radar plot showing the relationship between ARPC5 and tumor mutation burden (TMB). (C) Radar plot showing the correlation of ARPC5 with microsatellite instability (MSI). (D) Radar plot showing the correlation of ARPC5 with neoantigens. The number in radar plot represents Spearman’s correlation coefficient. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing, Mutagenesis, Immunopeptidomics

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Correlation analysis between ARPC5 expression and RNA modification-related genes, DNA methyltransferases, and tumor stemness score in 33 cancer types. (A) Co-expression of ARPC5 with m1A-related genes. (B) Co-expression of ARPC5 with m5C-related genes. (C) Co-expression of ARPC5 with m6A-related genes. (D) Co-expression of ARPC5 with DNA methyltransferases. (E) The correlation between ARPC5 expression and Tumor Stemness score (DNAss). (F) The correlation between ARPC5 expression and Tumor Stemness score (RNAss). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing, RNA modification

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: ARPC5 is upregulated in HCC cells and primary HCC tissues. (A) qPCR analysis of ARPC5 mRNA expression in four HCC cell lines (MHCC97-H, Huh7, HCC-LM3, and HepG2) and normal liver cell line (LO2). GAPDH was used as an internal control error bars represent M ± SEM (triplicate experiments). (B, C) The protein expression of ARPC5 was detected in four HCC cell lines and normal liver cell line with Western blot analysis. Error bars represent M ± SD of triplicate measurements. (D) The mRNA expression of ARPC5 in 40 pairs HCC tissues and adjacent para-carcinoma tissues was evaluated using qPCR. (E) Western blot analysis of ARPC5 protein expression in 10 paired HCC tissues and adjacent normal tissues. The number presented the relative protein expression levels of ARPC5. (F) Representative images of ARPC5 immunohistochemical staining analysis in the HCC tissue and adjacent normal liver tissue, original magnifications: ×40 and ×200. Scale bars, 50 μm. (G) Quantitative analysis of ARPC5 expression in HCC tissues based on mean optical density of immunohistochemical staining. Error bars represent the M ± SD of multiple tissues. (H) Kaplan–Meier curves showed that higher expression of ARPC5 was associated with poor DFS in HCC patients. * p < 0.05; ** p < 0.01; *** p < 0.001. ns, no significance.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing, Control, Western Blot, Immunohistochemical staining, Staining

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Silencing of ARPC5 inhibits cell proliferation and promotes cell apoptosis of HCC. (A) The knockdown efficiency of siRNA–ARPC5 was examined in HCC-LM3 and MHCC97-H cells with qPCR. (B) The knockdown efficiency of siRNA-ARPC5 was examined in HCC-LM3 and MHCC97-H cells with Western blot. The number presented as relative protein expression levels of ARPC5. ( C–D ) EdU assays for HCC-LM3 and MHCC 97-H were performed to evaluate cell proliferation ability after transfecting siRNA-ARPC5#1. Representative images (C) and the number of proliferative cells were calculated (D) ; original magnification, ×200. (E–F) Cellular growth curves were evaluated by CCK-8 assays in HCC-LM3 and MHCC97-H cells. (G – H) Flow cytometry was applied to test the apoptosis of HCC cells transfected with si-ARPC5 #1 in HCC-LM3 and MHCC 97-H cells. All data are presented as the M ± SD of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001. ns, no significance.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Knockdown, Western Blot, Expressing, CCK-8 Assay, Flow Cytometry, Transfection

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet: Role of ARPC5 inhibition on migration, invasion, and epithelial–mesenchymal transition (EMT) of HCC cells. (A–D) Migration ability was assessed by scratch wound healing assay, representative images (A, B) were shown (original magnification, ×200; scale bars, 50 µm), and wound healing areas were calculated (C, D) . (E , F) Transwell assay was applied to examine the invasion ability, representative images (F) were shown (original magnification, ×200; scale bars, 50 µm), and the histogram showed the number of invasion cells (E) . (G) Western blot showed the changes of EMT proteins in HCC-LM3 and MHCC97-H cells transfected with si-ARPC5#1. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Inhibition, Migration, Wound Healing Assay, Transwell Assay, Western Blot, Transfection

Journal: Frontiers in Immunology

Article Title: A comprehensively prognostic and immunological analysis of actin-related protein 2/3 complex subunit 5 in pan-cancer and identification in hepatocellular carcinoma

doi: 10.3389/fimmu.2022.944898

Figure Lengend Snippet:

Article Snippet: After the antigen retrieval, the rabbit anti-human ARPC5(1:500, T553316S, Abmart) primary antibody was applied to the slides and incubated at 4°C overnight and followed by the secondary anti–horseradish peroxide for 30 min. Next, the slides were stained with DAB chromogenic reagent and hematoxylin.

Techniques: Expressing, Mutagenesis, Real-time Polymerase Chain Reaction, Small Interfering RNA